体外血栓模型设计及标准化探讨_《华西医学》

作者：

喻琴梅 ¹ , 周翔 ² ,  牛挺 ¹

四川大学华西医院（成都，610041）1血液科及血液学研究室，2超声诊断科;

关键词：

溶栓体外血栓血栓模型标准化

DOI：

CNKI: 51-1356/R.20120214.1340.010

视频：

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针对栓塞血管及时、快速溶栓是治疗心脑、肢体等血管血栓性疾病的关键之一。但溶栓过程中的出血和再栓塞等问题始终未得到很好的解决，仍需要大量的体外溶栓实验研究。体外血栓模型的设计和完善是每个体外实验研究的必需技术，且直接影响到溶栓效果的评价，但目前国内外尚缺乏统一、标准的模型设计。现就近年来文献报道的常用体外血栓形成模型及其标准化进行探讨。

引用本文： 喻琴梅,周翔,牛挺. 体外血栓模型设计及标准化探讨. 华西医学, 2012, 27(2): 210-212. doi: CNKI: 51-1356/R.20120214.1340.010 复制

1.	Holland CK, Vaidya SS, Datta S, et al. Ultrasound-enhanced tissue plasminogen activator thrombolysis in an invitro porcine clot model[J]. Thromb Res, 2008, 121(5): 663-673.
2.	Shaw GJ, Bavani N, Dhamija A, et al. Effect of mild hypothermia on the thrombolytic efficacy of 120 kHz ultrasound enhanced thrombolysis in an in-vitro human clot model[J]. Thromb Res, 2006, 117(5): 603-608.
3.	Meretoja A, Tatlisumak T. Thrombolytic therapy in acute ischemic stroke-basic concepts[J]. Curr Vasc Pharmacol, 2006, 4(1): 31-44.
4.	Esmon CT .Basic mechanisms and pathogenesis of venous thrombosis[J]. Blood Rev, 2009, 23(5): 225-229.
5.	Krueger K, Deissler P, Coburger S, et al. How thrombus model impacts the in vitro study of interventional thrombectomy procedures[J]. Invest Radiol, 2004, 39(10): 641-648.
6.	杨建军, 宋雪丹, 陈海英, 等. 家犬自体血栓性脑梗死模型的建立[J]. 东南国防医药, 2009, 11(3): 196-198.
7.	严碧歌. 血栓形成过程与其密度的相关性研究[J]. 西北大学学报·自然科学版, 2006, 36(3): 356-358.
8.	Kirchhof K, Welzel T, Zoubaa S, et al. New method of embolus preparation for standardized embolic stroke in rabbits[J]. Stroke, 2002, 33(9): 2329-2333.
9.	Grimm J, Jahnke T, Muhle C, et al. Influence of thrombus age on the mechanical thrombectomy efficacy of the amplatz thrombectomy device in vitro[J]. Cardiovasc Intervent Radiol, 2003, 26(3): 265-268.
10.	Grimm J, Müller-Hülsbeck S, Heller M. Comparison of the mechanical thrombectomy efficacy of the amplatz thrombectomy device and the cragg thrombolytic brush in vitro[J]. Invest Radiol, 2001, 36(4): 204-209.
11.	华兴, 高云华, 刘政, 等. 辐照时间与血栓凝龄对超声波体外溶栓的影响[J]. 临床超声医学杂志, 2007, 9(4): 193-195.
12.	杜宝琮, 杜宝民, 翟桂琴. 体外治疗量超声对血栓溶解效应的影响[J]. 中华物理医学与康杂志, 2002, 24(7): 417-420.
13.	徐亚丽, 高云华, 刘政, 等. 超声联合微泡溶解体外血栓声像图改变与病理对照研究[J]. 中国超声医学杂志, 2006, 22(3): 164-167.
14.	Terres W, Beythien C, Kupper W, et al. Effects of aspirin and prostaglandin E1 on in vitro thrombolysis with urokinase. Evidence for a possible role of inhibiting platelet activity in thrombolysis[J]. Circulation, 1989, 79(6): 1309-1314.
15.	Torno MD, Kaminski MD, Xie Y, et al. Improvement of in vitro thrombolysis employing magnetically-guided microspheres[J]. Thromb Res, 2008, 121(6): 799-811.
16.	Pfaffenberger S, Devcic-Kuhar B, Kollmann C, et al. Can a commercial diagnostic ultrasound device accelerate thrombolysis? An in vitro skull model[J]. Stroke, 2005, 36(1): 124-128.
17.	党京丹. 血栓与止血试验诊断的研究新进展[J]. 当代医学, 2009, 15(22): 13-14.
18.	Müller-Hülsbeck S, Grimm J, Suwelack D, et al. Mechanical assistance for enhancement of in vitro effectiveness for hydryodynamic thrombectomy [J]. Invest Radiol, 1999, 34(11): 699-677.

1. Holland CK, Vaidya SS, Datta S, et al. Ultrasound-enhanced tissue plasminogen activator thrombolysis in an invitro porcine clot model[J]. Thromb Res, 2008, 121(5): 663-673.
2. Shaw GJ, Bavani N, Dhamija A, et al. Effect of mild hypothermia on the thrombolytic efficacy of 120 kHz ultrasound enhanced thrombolysis in an in-vitro human clot model[J]. Thromb Res, 2006, 117(5): 603-608.
3. Meretoja A, Tatlisumak T. Thrombolytic therapy in acute ischemic stroke-basic concepts[J]. Curr Vasc Pharmacol, 2006, 4(1): 31-44.
4. Esmon CT .Basic mechanisms and pathogenesis of venous thrombosis[J]. Blood Rev, 2009, 23(5): 225-229.
5. Krueger K, Deissler P, Coburger S, et al. How thrombus model impacts the in vitro study of interventional thrombectomy procedures[J]. Invest Radiol, 2004, 39(10): 641-648.
6. 杨建军, 宋雪丹, 陈海英, 等. 家犬自体血栓性脑梗死模型的建立[J]. 东南国防医药, 2009, 11(3): 196-198.
7. 严碧歌. 血栓形成过程与其密度的相关性研究[J]. 西北大学学报·自然科学版, 2006, 36(3): 356-358.
8. Kirchhof K, Welzel T, Zoubaa S, et al. New method of embolus preparation for standardized embolic stroke in rabbits[J]. Stroke, 2002, 33(9): 2329-2333.
9. Grimm J, Jahnke T, Muhle C, et al. Influence of thrombus age on the mechanical thrombectomy efficacy of the amplatz thrombectomy device in vitro[J]. Cardiovasc Intervent Radiol, 2003, 26(3): 265-268.
10. Grimm J, Müller-Hülsbeck S, Heller M. Comparison of the mechanical thrombectomy efficacy of the amplatz thrombectomy device and the cragg thrombolytic brush in vitro[J]. Invest Radiol, 2001, 36(4): 204-209.
11. 华兴, 高云华, 刘政, 等. 辐照时间与血栓凝龄对超声波体外溶栓的影响[J]. 临床超声医学杂志, 2007, 9(4): 193-195.
12. 杜宝琮, 杜宝民, 翟桂琴. 体外治疗量超声对血栓溶解效应的影响[J]. 中华物理医学与康杂志, 2002, 24(7): 417-420.
13. 徐亚丽, 高云华, 刘政, 等. 超声联合微泡溶解体外血栓声像图改变与病理对照研究[J]. 中国超声医学杂志, 2006, 22(3): 164-167.
14. Terres W, Beythien C, Kupper W, et al. Effects of aspirin and prostaglandin E1 on in vitro thrombolysis with urokinase. Evidence for a possible role of inhibiting platelet activity in thrombolysis[J]. Circulation, 1989, 79(6): 1309-1314.
15. Torno MD, Kaminski MD, Xie Y, et al. Improvement of in vitro thrombolysis employing magnetically-guided microspheres[J]. Thromb Res, 2008, 121(6): 799-811.
16. Pfaffenberger S, Devcic-Kuhar B, Kollmann C, et al. Can a commercial diagnostic ultrasound device accelerate thrombolysis? An in vitro skull model[J]. Stroke, 2005, 36(1): 124-128.
17. 党京丹. 血栓与止血试验诊断的研究新进展[J]. 当代医学, 2009, 15(22): 13-14.
18. Müller-Hülsbeck S, Grimm J, Suwelack D, et al. Mechanical assistance for enhancement of in vitro effectiveness for hydryodynamic thrombectomy [J]. Invest Radiol, 1999, 34(11): 699-677.

《华西医学》

体外血栓模型设计及标准化探讨

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《华西医学》

体外血栓模型设计及标准化探讨

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